rabbit anti igfbp6 polyclonal antibody  (Santa Cruz Biotechnology)

Journal: Cancer Immunology, Immunotherapy

Article Title: Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

doi: 10.1007/s00262-022-03215-3

Figure Lengend Snippet: Lactate induces IGFBP6 expression which promotes M2-like phenotype polarization of microglia. Effect of lactate on IGFBP6 A mRNA expression levels, B protein expression and C production in microglia cells. Evaluation of relative mRNA expression levels of D ATP5F1A , E COX IV , F COX II , G CyTB , H ND4 . I Cytoplasm Mitotracker Intensity. Evaluation of relative mRNA expression levels of J Arg1, K CD 206 , L CD 163 and M TNF , analyzed by real-time PCR. The calculated value of 2 − ΔΔ Ct in untreated controls is 1. Immunocytochemistry analysis of N iNOS and O Arg1, following 72 h of IGFBP6 treatment. Data are expressed as mean ± SD of at least four independent experiments. (* P < 0.05; ** P < 0.005; *** P < 0.001; **** P < 0.0001). Scale bars in ( I, N and O ) 10 μm

Article Snippet: Then, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-IGFBP6 antibody (Sigma, Milan, Italy), ready to use in PBS (Sigma, Milan, Italy) and MIB-1, a monoclonal antibody directed against the Ki-67 antigen (M7240; Dako Corporation, Glostrup, Denmark), diluted 1:75 in PBS (Sigma, Milan, Italy).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunocytochemistry

Journal: Cancer Immunology, Immunotherapy

Article Title: Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

doi: 10.1007/s00262-022-03215-3

Figure Lengend Snippet: Lactate and IGFBP6 crosstalk. Effect of lactate on IGFBP6 A mRNA expression levels and B production in Glioblastoma cells. Effect of IGFBP6 treatment on lactate production in ( C ) U-87 MG, E A-172 and G U-251 MG cells. Evaluation of mRNA expression levels of LDHA, ENO1 and HK2 in D U-87 MG, F A-172 and H U-251 MG cells

Article Snippet: Then, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-IGFBP6 antibody (Sigma, Milan, Italy), ready to use in PBS (Sigma, Milan, Italy) and MIB-1, a monoclonal antibody directed against the Ki-67 antigen (M7240; Dako Corporation, Glostrup, Denmark), diluted 1:75 in PBS (Sigma, Milan, Italy).

Techniques: Expressing

Journal: Cancer Immunology, Immunotherapy

Article Title: Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

doi: 10.1007/s00262-022-03215-3

Figure Lengend Snippet: IGFBP6 enhances migratory and colony formation capacity in glioblastoma cells. Effect of IGFBP6 treatment on migratory capacity A – D and colony formation capacity G – H in Glioblastoma cells. Data are expressed as mean ± SD of at least four independent experiments. (* P < 0.05; ** P < 0.005; *** P < 0.001; **** P < 0.0001). Scale bar in ( A ) 100 μm and scale bar in ( E , F and G ) 1 mm

Article Snippet: Then, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-IGFBP6 antibody (Sigma, Milan, Italy), ready to use in PBS (Sigma, Milan, Italy) and MIB-1, a monoclonal antibody directed against the Ki-67 antigen (M7240; Dako Corporation, Glostrup, Denmark), diluted 1:75 in PBS (Sigma, Milan, Italy).

Techniques:

Journal: Cancer Immunology, Immunotherapy

Article Title: Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

doi: 10.1007/s00262-022-03215-3

Figure Lengend Snippet: IGFBP6 increases sonic hedgehog signalling pathway activation in GBM and microglia.Evaluation of mRNA expression levels of SHH in microglia cells treated with IGFBP6 A , and GLI1 protein expression after IGFBP6 and cyclopamine treatment B . Evaluation of mRNA expression levels of TLR4 C and ARG1 D after IGFBP6 and cyclopamine treatment in microglia cells. Evaluation of mRNA expression levels of TLR4 after IGFBP6 and cyclopamine treatment in U-87 MG E , A-172 F and U-251 MG G cells. Effect of IGFBP6 and cyclopamine treatment on lactate production in U-87 MG H , A-172 I and U-251 MG J cells. Data are expressed as mean ± SD of at least four independent experiments. (* P < 0.05; ** P < 0.005; *** P < 0.001; **** P < 0.0001)

Article Snippet: Then, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-IGFBP6 antibody (Sigma, Milan, Italy), ready to use in PBS (Sigma, Milan, Italy) and MIB-1, a monoclonal antibody directed against the Ki-67 antigen (M7240; Dako Corporation, Glostrup, Denmark), diluted 1:75 in PBS (Sigma, Milan, Italy).

Techniques: Activation Assay, Expressing

Journal: Cancer Immunology, Immunotherapy

Article Title: Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

doi: 10.1007/s00262-022-03215-3

Figure Lengend Snippet: IGFBP6 produced by glioblastoma cells promote microglia M2-like phenotype polarization.Evaluation of mRNA expression levels of TNF, IL1β, ARG1, CD206 and CD163 in microglia cells treated with conditioned medium of A-B U-87 MG, C-D A-172, E-F U-251 MG pre-treated with IGFBP6. Data are expressed as mean ± SD of at least four independent experiments. (* P < 0.05; ** P < 0.005; *** P < 0.001; **** P < 0.0001)

Article Snippet: Then, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-IGFBP6 antibody (Sigma, Milan, Italy), ready to use in PBS (Sigma, Milan, Italy) and MIB-1, a monoclonal antibody directed against the Ki-67 antigen (M7240; Dako Corporation, Glostrup, Denmark), diluted 1:75 in PBS (Sigma, Milan, Italy).

Techniques: Produced, Expressing

Journal: Cancer Immunology, Immunotherapy

Article Title: Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

doi: 10.1007/s00262-022-03215-3

Figure Lengend Snippet: IGFBP6 was modulated in GBM patients.Analysis of IGFBP6 gene expression in brain biopsies of patients with astrocytoma, oligodendrocytoma, glioblastoma, and healthy subjects. b Pearson correlation analysis between IGFBP6 expression levels and tumor grade of brain biopsies obtained from patients affected by main brain tumors. c Pearson's correlation between IGFBP6 and IDH1 expression levels in brain biopsies of patients with glioblastoma. d Receiver operating characteristic (ROC) analysis between IGFBP6 brain expression levels in healthy subjects vs glioblastoma patients, between glioblastoma patients vs astrocytoma patients e , and vs oligodendrocytoma f . Data are expressed as mean ± SD of at least four independent experiments. (* P < 0.05; ** P < 0.005; *** P < 0.001; **** P < 0.0001). Scale bar in A 50 μm

Article Snippet: Then, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-IGFBP6 antibody (Sigma, Milan, Italy), ready to use in PBS (Sigma, Milan, Italy) and MIB-1, a monoclonal antibody directed against the Ki-67 antigen (M7240; Dako Corporation, Glostrup, Denmark), diluted 1:75 in PBS (Sigma, Milan, Italy).

Techniques: Expressing